Why do we Plasmids for genetic engineering instead of the chromosomse OR DNA?

[JackyM]

THX

[remaydnas]

we use plasmids 4 genetic engineering becuz bacteria replicate very fast and do not have complex organelles and things like that.

[CultureNine6995]

Plasmids are used because:- They are separate from the chromosomal DNA so they can be extracted easily- Unlike chromosomal DNA which codes for proteins necessary for cell survival. Plasmids don't have many genes, so it is easier to get the genes you want without having to isolate it from many other genes.- They are generally smaller in size, so they won't use much cellular resources to replicate, thus multiple copies can be inserted into a single cell.

[dineshg]

Reasons for using Plasmids for Genetic engineering 1)Plasmids are circular extrachromosomal DNA Molecules that are capable of autonomous replication .2)Number of plasmids in Bacteria or yeast is high (copy number is high )3) Isolation and modification of Plasmids is easy .4) Insertion of desired gene and later transformation of recombinant Plasmids to bacteris is easy .5)Screening of Bacteria or yeast containing recombinant plasmid is easy.

[Pierian]

More and more we do use chromosomes. Artificial chromosomes for bacteria, yeast and even humans are becoming common. Unlike plasmids, which seldom carry more than a few genes, and may be lost from the cell at any time, chromosomes can carry an unlimited number of genes and are generally stable within the cell line.